Composite

Part:BBa_K4683024:Experience

Designed by: Sahana Narayanan, Kate Sharer   Group: iGEM23_Lambert-GA   (2023-10-12)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa K4683024

Lambert_GA 2023
Gel Experimentation
We performed RCA on three miRNAs (miR-20b, miR-328,miR-146) and ran the subsequent rolling circle product (RCP) on a 1% agarose gel (see Fig.). The gel exhibited a fluorescent band of DNA very close to the well, indicating that a long strand of DNA greater than 1 kB - our RCP - was produced. Therefore, we can validate that our reaction was successful.
Figure 1. Image of gel ran with miRNA-1 RCP product; A: eRCA with 40.8 pM miR-1; B: negative control (no enzymes)
Figure. RCP from RCA reaction with miR-20b, miR-328,miR-146a; visible bands can be seen near the top of the wells.


User Reviews

UNIQb31ce3acf7b6f66a-partinfo-00000001-QINU UNIQb31ce3acf7b6f66a-partinfo-00000002-QINU